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Purpose(Empty Backbone) Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98558 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCB301
- Backbone size (bp) 4500
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Modifications to backboneGenerate StuI site at 35S Promoter transcription start site, insert SmaI site and Ribozyme from Hepatitis Delta Virus upstream of 35S terminator.
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Vector typePlant Expression
- Promoter Enh 35S
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJL 89 was a gift from John Lindbo (Addgene plasmid # 98558 ; http://n2t.net/addgene:98558 ; RRID:Addgene_98558) -
For your References section:
TRBO: a high-efficiency tobacco mosaic virus RNA-based overexpression vector. Lindbo JA. Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24. 10.1104/pp.107.106377 PubMed 17720752
Map uploaded by the depositor.
