BB1_12_pAOX1
(Plasmid #98514)

• Purpose: BB1 with insert: promoter PAOX1
• Depositing Labs: Brigitte Gasser, Diethard Mattanovich, Michael Sauer
• Publication: Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.

Backbone

• Vector backbone: BB1
• Backbone size w/o insert (bp): 1936
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AOX1 promoter
• Insert Size (bp): 928
• Mutation: Internal BsaI and BpiI sites removed from insert

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BpiI (destroyed during cloning)
• 3′ cloning site: BpiI (destroyed during cloning)
• 5′ sequencing primer: 5'-GTAAAACGACGGCCAGTT-3' (M13-Fwd)
• 3′ sequencing primer: 5´-CAGGAAACAGCTATGAC-3' (M13-Rev)

Resource Information

• Supplemental Documents:
  • BB1_12_pAOX1 Genbank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that some discrepancies in the RPL2A promoter sequence were found during Addgene's Quality Control Process. The depositing lab noted that these discrepancies are likely due to errors in the initial genome sequence and should NOT affect the function of the plasmid.