BB1_12_pRPL2A
(Plasmid #98508)

• Purpose: BB1 with insert: promoter PRPL2A
• Depositing Labs: Brigitte Gasser, Diethard Mattanovich, Michael Sauer
• Publication: Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.

Backbone

• Vector backbone: BB1
• Backbone size w/o insert (bp): 1936
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RPL2A promoter
• Insert Size (bp): 949
• Mutation: Internal BsaI and BpiI sites removed from insert

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BpiI (destroyed during cloning)
• 3′ cloning site: BpiI (destroyed during cloning)
• 5′ sequencing primer: 5'-GTAAAACGACGGCCAGTT-3' (M13-Fwd)
• 3′ sequencing primer: 5´-CAGGAAACAGCTATGAC-3' (M13-Rev)

Resource Information

• Supplemental Documents:
  • BB1_12_pRPL2A Genbank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that 1 mismatch ( bp1613 a>g) in the RPL2A promoter sequence was found during Addgene's Quality Control Process. The depositing lab noted that this mismatch is likely due to errors in the initial genome sequence and should NOT affect the function of the plasmid.