pYZ149
(Plasmid #98407)

• Purpose: Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.
• Depositing Lab: Jef Boeke
• Publication: Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.

Backbone

• Vector backbone: pAV10
• Backbone size w/o insert (bp): 2940
• Vector type: CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: upstream homologous region to delete his3 in genome by recombination
• Alt name: alg2
• Species: S. pombe (fission yeast)
• Insert Size (bp): 2076
• Entrez Gene: alg2 (a.k.a. SPBC11B10.01, SPBC32H8.14)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Gene/Insert 2

• Gene/Insert name: downstream homologous region to delete his3 in genome by recombination
• Alt name: cog8
• Species: S. pombe (fission yeast)
• Entrez Gene: cog8 (a.k.a. SPBC11B10.03)

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pYZ149 his3 donor template for complete deletion.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry