pYZ145
(Plasmid #98405)

• Purpose: Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.
• Depositing Lab: Jef Boeke
• Publication: Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.

Backbone

• Vector backbone: pAV10
• Backbone size w/o insert (bp): 2940
• Vector type: CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: upstream homologous region to delete leu1 in genome by recombination
• Alt name: apc2
• Species: S. pombe (fission yeast)
• Insert Size (bp): 2076
• Entrez Gene: apc2 (a.k.a. SPBP23A10.04)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Gene/Insert 2

• Gene/Insert name: downstream homologous region to delete leu1 in genome by recombination
• Alt name: top2
• Species: S. pombe (fission yeast)
• Entrez Gene: top2 (a.k.a. SPBC1A4.03c, ptr11)

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pYZ145 leu1 donor DNA rpl301 spacer for complete genome deletion.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry