pBabe NEO mRFP1
(Plasmid #98397)

• Purpose: Retroviral vector for constitutive mRFP1 expression
• Depositing Lab: Kevin Janes
• Publication: Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027.

Backbone

• Vector backbone: pBABE-neo
• Backbone manufacturer: Addgene #1767
• Total vector size (bp): 6059
• Modifications to backbone: XhoI site added immediately behind Venus sequence
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mRFP1
• Insert Size (bp): 681

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned from pRSET-mRFP (http://www.pnas.org/content/99/12/7877.full)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Retroviral vector for mRFP1 expression