pSLIK TT 3xFLAG ID2 neo
(Plasmid #98393)

• Purpose: Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag
• Depositing Lab: Kevin Janes
• Publication: Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027.

Backbone

• Vector backbone: pSLIK-neo
• Backbone manufacturer: Addgene #25735
• Backbone size w/o insert (bp): 13286
• Total vector size (bp): 12491
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ID2
• Species: H. sapiens (human)
• Insert Size (bp): 402
• GenBank ID: NM_002166
• Entrez Gene: ID2 (a.k.a. GIG8, ID2A, ID2H, bHLHb26)
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hUBCpro-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lentiviral mammalian expression plasmid for inducible expression of 3xFLAG ID2 with neomycin selection; recombined from Addgene #98390 and Addgene #25735.