pSLIK TT 3xFLAG Luciferase neo
(Plasmid #98392)

• Purpose: Lentiviral vector for inducible expression of Luciferase with 3xFLAG N-terminal tag
• Depositing Lab: Kevin Janes
• Publication: Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027.

Backbone

• Vector backbone: pSLIK-neo
• Backbone manufacturer: Addgene #25735
• Backbone size w/o insert (bp): 13286
• Total vector size (bp): 13848
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Luciferase
• Insert Size (bp): 1644
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hUBCpro-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lentiviral mammalian expression plasmid for inducible expression of 3xFLAG Luciferase with neomycin selection; recombined from Addgene #98391 and Addgene #25735.