pEN_TT 3xFLAG ID2
(Plasmid #98390)

• Purpose: Gateway entry vector for inducible expression of human ID2 with N-terminal 3xFLAG tag
• Depositing Lab: Kevin Janes
• Publication: Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027.

Backbone

• Vector backbone: pEN_TTmiRc2
• Backbone manufacturer: Addgene #83274
• Backbone size w/o insert (bp): 4496
• Total vector size (bp): 4257
• Modifications to backbone: 3xFLAG cloned in frame before restriction sites
• Vector type: entry vector for gateway cloning

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ID2
• Species: H. sapiens (human)
• Insert Size (bp): 402
• GenBank ID: NM_002166
• Entrez Gene: ID2 (a.k.a. GIG8, ID2A, ID2H, bHLHb26)
• Promoter: TRE tight promoter
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (unknown if destroyed)
• 3′ cloning site: MfeI (unknown if destroyed)
• 5′ sequencing primer: pCEP-fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PCR cloned from Thermo Open Biosystems Human ORFeome version 5.1 #1362

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gateway entry vector for expression of 3xFLAG ID2 (human)