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Addgene

pIMVAd40R
(Plasmid #98309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS424
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1<ERG8<PGAL10-PGAL1-IDI1-TRPL15A-loxP-ble-loxP-TERG9
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please set 1/1/2018 as the initial day available for public access. Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic seq

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIMVAd40R was a gift from Claudia Vickers (Addgene plasmid # 98309 ; http://n2t.net/addgene:98309 ; RRID:Addgene_98309)

  • For your References section:

    Engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in Saccharomyces cerevisiae. Peng B, Nielsen LK, Kampranis SC, Vickers CE. Metab Eng. 2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 10.1016/j.ymben.2018.02.005. 10.1016/j.ymben.2018.02.005 PubMed 29471044
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