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Addgene

pJT10R
(Plasmid #98307)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98307 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS425
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRPL3<ScERG20 N127W<PGAL1-PGAL2>AcNES1>TRPL41B
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    ERG20 N127W

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please set 1/1/2018 as the initial day available for public access. Yeast promoters and terminators were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genom

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT10R was a gift from Claudia Vickers (Addgene plasmid # 98307 ; http://n2t.net/addgene:98307 ; RRID:Addgene_98307)

  • For your References section:

    Engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in Saccharomyces cerevisiae. Peng B, Nielsen LK, Kampranis SC, Vickers CE. Metab Eng. 2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 10.1016/j.ymben.2018.02.005. 10.1016/j.ymben.2018.02.005 PubMed 29471044
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