pJT2
(Plasmid
#98306)
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PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98306 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS425
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
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SpeciesS. cerevisiae (budding yeast)
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MutationERG20 N127W
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please set 1/1/2018 as the initial day available for public access. Yeast promoters and terminators were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genom
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJT2 was a gift from Claudia Vickers (Addgene plasmid # 98306 ; http://n2t.net/addgene:98306 ; RRID:Addgene_98306) -
For your References section:
Engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in Saccharomyces cerevisiae. Peng B, Nielsen LK, Kampranis SC, Vickers CE. Metab Eng. 2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 10.1016/j.ymben.2018.02.005. 10.1016/j.ymben.2018.02.005 PubMed 29471044