pIMVAu1
(Plasmid
#98301)
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PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98301 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS423
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHMG2(-309,-153)-HIS3-TEFM1<EfmvaS<PGAL1-PGAL10>ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIMVAu1 was a gift from Claudia Vickers (Addgene plasmid # 98301 ; http://n2t.net/addgene:98301 ; RRID:Addgene_98301) -
For your References section:
Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast. Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE. Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 10.1186/s13068-017-0728-x. eCollection 2017. 728 [pii] PubMed 28239415