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Addgene

pJT1
(Plasmid #98299)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98299 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS425
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT1 was a gift from Claudia Vickers (Addgene plasmid # 98299 ; http://n2t.net/addgene:98299 ; RRID:Addgene_98299)

  • For your References section:

    A squalene synthase protein degradation method for improved sesquiterpene production in Saccharomyces cerevisiae. Peng B, Plan MR, Chrysanthopoulos P, Hodson MP, Nielsen LK, Vickers CE. Metab Eng. 2017 Jan;39:209-219. doi: 10.1016/j.ymben.2016.12.003. Epub 2016 Dec 8. 10.1016/j.ymben.2016.12.003 PubMed 27939849
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