pcDNA3.1-mNeonGreen-LEHD-NanoLuc
(Plasmid #98289)

• Purpose: caspase-9 BRET Sensor
• Depositing Lab: Maarten Merkx
• Publication: den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi: 10.1021/acssensors.7b00239. Epub 2017 May 31.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Genscript
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6781
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mNeonGreen-LEHD-NanoLuc
• Species: H. sapiens (human)
• Insert Size (bp): 1353
• Promoter: CMV
• Tag / Fusion Protein:
  • His-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-fwd
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ordered at Genscript
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry