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Addgene

mNeonGreen-IETD-NanoLuc
(Plasmid #98288)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98288 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6781
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mNeonGreen-LEHD-NanoLuc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1353
  • Promoter CMV
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-fwd
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ordered at Genscript

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mNeonGreen-IETD-NanoLuc was a gift from Maarten Merkx (Addgene plasmid # 98288 ; http://n2t.net/addgene:98288 ; RRID:Addgene_98288)

  • For your References section:

    Bright Bioluminescent BRET Sensor Proteins for Measuring Intracellular Caspase Activity. den Hamer A, Dierickx P, Arts R, de Vries JSPM, Brunsveld L, Merkx M. ACS Sens. 2017 Jun 23;2(6):729-734. doi: 10.1021/acssensors.7b00239. Epub 2017 May 31. 10.1021/acssensors.7b00239 PubMed 28670623
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