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Purposecaspase-3 BRET Sensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98287 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerGenscript
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6781
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeonGreen-LEHD-NanoLuc
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SpeciesH. sapiens (human)
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Insert Size (bp)1353
- Promoter CMV
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Tag
/ Fusion Protein
- His-tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-fwd
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byOrdered at Genscript
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mNeonGreen-DEVD-NanoLuc was a gift from Maarten Merkx (Addgene plasmid # 98287 ; http://n2t.net/addgene:98287 ; RRID:Addgene_98287) -
For your References section:
Bright Bioluminescent BRET Sensor Proteins for Measuring Intracellular Caspase Activity. den Hamer A, Dierickx P, Arts R, de Vries JSPM, Brunsveld L, Merkx M. ACS Sens. 2017 Jun 23;2(6):729-734. doi: 10.1021/acssensors.7b00239. Epub 2017 May 31. 10.1021/acssensors.7b00239 PubMed 28670623