pmEOS2-VSVG
(Plasmid
#98286)
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Purposemammalian expression of VSVG-mEOS2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98286 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPAGFP-VSVG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVSVG
- Promoter CMV
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Tag
/ Fusion Protein
- mEos2 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmEOS2-VSVG was a gift from Mike Heilemann (Addgene plasmid # 98286 ; http://n2t.net/addgene:98286 ; RRID:Addgene_98286) -
For your References section:
One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy. Fricke F, Beaudouin J, Eils R, Heilemann M. Sci Rep. 2015 Sep 11;5:14072. doi: 10.1038/srep14072. 10.1038/srep14072 PubMed 26358640