pirespuro2-CTLA4-mEos2
(Plasmid #98285)

• Purpose: mammalian expression of CTLA4-mEos2
• Depositing Lab: Mike Heilemann
• Publication: Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.1038/srep14072.

Backbone

• Vector backbone: pIRESpuro2
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CTLA4
• Species: H. sapiens (human)
• Entrez Gene: CTLA4 (a.k.a. ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEos2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

From depositor: The last 23 amino acids of CTLA-4 were
removed by PCR to reduce the internalization of the receptor and
concentrate it at the plasma membrane.