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pirespuro2-CD86-mEos2
(Plasmid #98284)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98284 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESpuro2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD86
  • Species
    H. sapiens (human)
  • Entrez Gene
    CD86 (a.k.a. B7-2, B7.2, B70, CD28LG2, LAB72)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEos2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pirespuro2-CD86-mEos2 was a gift from Mike Heilemann (Addgene plasmid # 98284 ; http://n2t.net/addgene:98284 ; RRID:Addgene_98284)

  • For your References section:

    One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy. Fricke F, Beaudouin J, Eils R, Heilemann M. Sci Rep. 2015 Sep 11;5:14072. doi: 10.1038/srep14072. 10.1038/srep14072 PubMed 26358640
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