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Addgene

pNup133-Ypet
(Plasmid #98283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    plasmid PAtagRFP-actin (Verkhusha lab/Addgene)
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nup133
  • Species
    H. sapiens (human)
  • Entrez Gene
    NUP133 (a.k.a. GAMOS8, NPHS18, hNUP133)
  • Promoter CMV
  • Tag / Fusion Protein
    • YPet (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ypet = fast maturing YFP variant

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNup133-Ypet was a gift from Mike Heilemann (Addgene plasmid # 98283 ; http://n2t.net/addgene:98283 ; RRID:Addgene_98283)

  • For your References section:

    A set of homo-oligomeric standards allows accurate protein counting. Finan K, Raulf A, Heilemann M. Angew Chem Int Ed Engl. 2015 Oct 5;54(41):12049-52. doi: 10.1002/anie.201505664. Epub 2015 Aug 20. 10.1002/anie.201505664 PubMed 26289028
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