PEP119T
(Plasmid
#98251)
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PurposePseudomonas T3SS gateway vector with HA-sfGFP11-sfGFP11
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98251 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBBR1-2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGateway cassettee-HA-sfGFP11-11
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PEP119T was a gift from Savithramma Dinesh-Kumar (Addgene plasmid # 98251 ; http://n2t.net/addgene:98251 ; RRID:Addgene_98251) -
For your References section:
Spatiotemporal Monitoring of Pseudomonas syringae Effectors via Type III Secretion Using Split Fluorescent Protein Fragments. Park E, Lee HY, Woo J, Choi D, Dinesh-Kumar SP. Plant Cell. 2017 Jul;29(7):1571-1584. doi: 10.1105/tpc.17.00047. Epub 2017 Jun 14. 10.1105/tpc.17.00047 PubMed 28619883