Skip to main content
Addgene

IPCEF1 (PIP3EA-c002)
(Plasmid #98240)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98240 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Backbone manufacturer
    Opher Gileadi (Addgene plasmid # 26103)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PIP3EA
  • Alt name
    IPCEF1
  • Alt name
    interaction protein for cytohesin exchange factors 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    IPCEF1 (a.k.a. PIP3-E)
  • Promoter T7
  • Tag / Fusion Protein
    • His-6-TEV (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use BL21(DE3)-R3-pRARE2 for expression. T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. 5MR1: http://www.thesgc.org/structures/5MR1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IPCEF1 (PIP3EA-c002) was a gift from Nicola Burgess-Brown (Addgene plasmid # 98240 ; http://n2t.net/addgene:98240 ; RRID:Addgene_98240)
Related items: