RECQL5 (RECQL5A-c104)
(Plasmid
#98234)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNIC28-Bsa4
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Backbone manufacturerOpher Gileadi (Addgene plasmid # 26103)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRECQL5A
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Alt nameRECQL5
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Alt nameRecQ like helicase 5
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SpeciesH. sapiens (human)
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Mutationpfam00270:DEAD 29-203, pfam00271:Helicase_C 251-327
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Entrez GeneRECQL5 (a.k.a. RECQ5)
- Promoter T7
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Tag
/ Fusion Protein
- His-6-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use BL21(DE3)-R3-pRARE2 for expression. T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone.. 5LB3: http://www.thesgc.org/structures/5LB3 5LBA: http://www.thesgc.org/structures/5LBA
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RECQL5 (RECQL5A-c104) was a gift from Nicola Burgess-Brown (Addgene plasmid # 98234 ; http://n2t.net/addgene:98234 ; RRID:Addgene_98234)
