PKD2 (PKD2A-c034)
(Plasmid
#98226)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFB-CT10HF-LIC
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Backbone manufacturerNicola Burgess-Brown (Addgene plasmid # 39191)
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Vector typeBaculovirus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePKD2A
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Alt namePKD2
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Alt namepolycystic kidney disease 2 (autosomal dominant)
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SpeciesH. sapiens (human)
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Mutationpfam00520:Ion_trans 475-680
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Entrez GenePKD2 (a.k.a. APKD2, PC2, PKD4, Pc-2, TRPP2)
- Promoter polyhedrin
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Tag
/ Fusion Protein
- TEV-His10-Flag (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Fbac-1
- 3′ sequencing primer Fbac-2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Baculo vector with C-terminal 10His+flag, TEV-cleavable, for LIC cloning. 5K47: http://www.thesgc.org/structures/5K47
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PKD2 (PKD2A-c034) was a gift from Nicola Burgess-Brown (Addgene plasmid # 98226 ; http://n2t.net/addgene:98226 ; RRID:Addgene_98226)
