NUDT17 (NUDT17A-c014)
(Plasmid
#98224)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98224 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFB-6HZB
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Vector typeBaculovirus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNUDT17A
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Alt nameNUDT17
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Alt namenudix (nucleoside diphosphate linked moiety X)-type motif 17
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SpeciesH. sapiens (human)
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Mutationpfam00293:NUDIX 93-235
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Entrez GeneNUDT17
- Promoter polyhedrin promoter
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Tag
/ Fusion Protein
- His6-ZBasic-TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Baculovirus (Bac to bac). N-terminal His6-ZB-TEV tag. Clone using BseRI and standard Lic extensions. 5LF8: http://www.thesgc.org/structures/5LF8
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NUDT17 (NUDT17A-c014) was a gift from Nicola Burgess-Brown (Addgene plasmid # 98224 ; http://n2t.net/addgene:98224 ; RRID:Addgene_98224)