-
PurposeVector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tag
-
Depositing Lab
-
Publication
-
Sequence Information
-
Sequences (2) — Accept Affinity Reagent Sequence Policy
-
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98223 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepMES4
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameXXBLMA
-
Alt nameXXBLMA
-
Alt nameBLM-nanobody complex
-
SpeciesH. sapiens (human)
-
Entrez GeneBLM (a.k.a. BS, MGRISCE1, RECQ2, RECQL2, RECQL3)
-
Tag
/ Fusion Protein
- His-6 (C terminal on backbone)
Cloning Information
- Cloning method Unknown
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use WK6 cells for expression. Vector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tag. 4CDG: https://www.thesgc.org/structures/4CDG.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
XXBLM (XXBLMA-c075) was a gift from Nicola Burgess-Brown (Addgene plasmid # 98223 ; http://n2t.net/addgene:98223 ; RRID:Addgene_98223)
