pAAV hSyn-Flex RG-cerulean
(Plasmid #98221)

• Purpose: Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus
• Depositing Lab: Troy Margrie
• Publication: SADB19 rabies virus tracing plasmid (unpublished)

Backbone

• Vector backbone: pAAV Ef1a flex C-RG
• Backbone size w/o insert (bp): 4223
• Total vector size (bp): 6590
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cerulean-E2A-RG
• Alt name: rabies virus glycoprotein
• Species: rabies virus
• Insert Size (bp): 2367
• Promoter: human Synapsin
• Tag / Fusion Protein:
  • cerulean (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: gaccatctgcgctgcggcg
• 3′ sequencing primer: CAAGGCTGGTGGGCACTGG

Resource Information

• Supplemental Documents:
  • pAAVsynFlexRGC-W.gb
• A portion of this plasmid was derived from a plasmid made by: I used the Addgene plasmid 26197 to originally amplify the RG portion of the insert
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry