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Addgene

UKNP3.1.2
(Plasmid #98215)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98215 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HCV E1E2
  • Alt name
    UKNP3.1.2
  • Species
    Hepatitis C virus
  • Insert Size (bp)
    1752
  • GenBank ID
    KU285215.1

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The HCV E1E2 insert contains W500L and C549A mutations compared to KU285215.1 but the depositor has confirmed that they do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UKNP3.1.2 was a gift from Jonathan Ball (Addgene plasmid # 98215 ; http://n2t.net/addgene:98215 ; RRID:Addgene_98215)

  • For your References section:

    A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in Vaccine Research Reveals Extremes of Monoclonal Antibody Neutralization Resistance. Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey T, Irving WL, Ball JK, Tarr AW. J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15. 10.1128/JVI.02700-15 PubMed 26699643
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