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Addgene

pCasSA
(Plasmid #98211)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98211 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Backbone size (bp) 10240
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Chloramphenicol (in S. aureus)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The plasmid causes the E. coli to grow very slowly at 30C and plates may need to be incubated for 24-36 hours in order to see colonies
  • Copy number
    High Copy

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCasSA was a gift from Quanjiang Ji (Addgene plasmid # 98211 ; http://n2t.net/addgene:98211 ; RRID:Addgene_98211)

  • For your References section:

    Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System. Chen W, Zhang Y, Yeo WS, Bae T, Ji Q. J Am Chem Soc. 2017 Mar 15;139(10):3790-3795. doi: 10.1021/jacs.6b13317. Epub 2017 Mar 2. 10.1021/jacs.6b13317 PubMed 28218837
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