PUC57_BAG3P209L-eGFP
(Plasmid
#98183)
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PurposeCodes for full length, GFP tagged mutant Bag3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98183 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePUC57
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBAG3P209L-eGFP
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SpeciesH. sapiens (human)
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MutationP209L
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Entrez GeneBAG3 (a.k.a. BAG-3, BIS, CAIR-1, MFM6)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PUC57_BAG3P209L-eGFP was a gift from Robert Bryson-Richardson (Addgene plasmid # 98183 ; http://n2t.net/addgene:98183 ; RRID:Addgene_98183) -
For your References section:
Zebrafish models of BAG3 myofibrillar myopathy suggest a toxic gain of function leading to BAG3 insufficiency. Ruparelia AA, Oorschot V, Vaz R, Ramm G, Bryson-Richardson RJ. Acta Neuropathol. 2014 Dec;128(6):821-33. doi: 10.1007/s00401-014-1344-5. Epub 2014 Oct 2. 10.1007/s00401-014-1344-5 PubMed 25273835