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Addgene

PUC57_BAG3P209L-eGFP
(Plasmid #98183)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98183 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PUC57
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAG3P209L-eGFP
  • Species
    H. sapiens (human)
  • Mutation
    P209L
  • Entrez Gene
    BAG3 (a.k.a. BAG-3, BIS, CAIR-1, MFM6)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PUC57_BAG3P209L-eGFP was a gift from Robert Bryson-Richardson (Addgene plasmid # 98183 ; http://n2t.net/addgene:98183 ; RRID:Addgene_98183)
  • For your References section:

    Zebrafish models of BAG3 myofibrillar myopathy suggest a toxic gain of function leading to BAG3 insufficiency. Ruparelia AA, Oorschot V, Vaz R, Ramm G, Bryson-Richardson RJ. Acta Neuropathol. 2014 Dec;128(6):821-33. doi: 10.1007/s00401-014-1344-5. Epub 2014 Oct 2. 10.1007/s00401-014-1344-5 PubMed 25273835