pBluescript-Maf
(Plasmid
#98179)
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PurposeExpression of a cMaf in situ hybridization probe
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98179 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript
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Vector typeIn situ hybridization
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecMaf, partial mRNA
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SpeciesM. musculus (mouse)
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Insert Size (bp)674
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Entrez GeneMaf (a.k.a. 2810401A20Rik, A230108G15Rik, AW047063, c-maf)
- Promoter T3/T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmids generates antisense and sense ISH probes. Antisense: by using T7 and digesting with NotI. Sense: by using T3 and digesting with KpnI
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBluescript-Maf was a gift from Oscar Marin (Addgene plasmid # 98179 ; http://n2t.net/addgene:98179 ; RRID:Addgene_98179) -
For your References section:
Optimization of interneuron function by direct coupling of cell migration and axonal targeting. Lim L, Pakan JMP, Selten MM, Marques-Smith A, Llorca A, Bae SE, Rochefort NL, Marin O. Nat Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 10.1038/s41593-018-0162-9. 10.1038/s41593-018-0162-9 PubMed 29915195
