p-mCherry-C1-iChR2++
(Plasmid #98172)

• Purpose: artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.
• Depositing Lab: Peter Hegemann
• Publication: Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.1038/s41598-017-14330-y.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4714
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Synthetic construct iChR2++ gene
• Alt name: iChR2++
• Alt name: CrChR2
• Species: Synthetic
• Insert Size (bp): 927
• Mutation: A59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V242R, T246N, N258Q, E273S
• Promoter: CMV (+enhancer)
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: EGFP-N1-F: GAGGTCTATATAAGCAGAGC or CMV-F: GCAAATGGGCGGTAGGCGT
• 3′ sequencing primer: EGFP-C1-R: AACCATTATAAGCTGCAATAAAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry