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Addgene

pJ414-PCY1:Y696G
(Plasmid #98156)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98156 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ414
  • Backbone manufacturer
    DNA2.0
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    200 rpm
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Peptide cyclase 1:Y696G
  • Alt name
    PCY1:Y696G
  • Species
    Saponaria vaccaria
  • Insert Size (bp)
    2175
  • Mutation
    Changed tyrosine 696 to glycine
  • Promoter T7
  • Tag / Fusion Protein
    • His6-TEV (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ414-PCY1:Y696G was a gift from James Naismith (Addgene plasmid # 98156 ; http://n2t.net/addgene:98156 ; RRID:Addgene_98156)

  • For your References section:

    Characterization of the Fast and Promiscuous Macrocyclase from Plant PCY1 Enables the Use of Simple Substrates. Ludewig H, Czekster CM, Oueis E, Munday ES, Arshad M, Synowsky SA, Bent AF, Naismith JH. ACS Chem Biol. 2018 Mar 16;13(3):801-811. doi: 10.1021/acschembio.8b00050. Epub 2018 Feb 12. 10.1021/acschembio.8b00050 PubMed 29377663
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