pJ414-PCY1
(Plasmid #98150)

• Purpose: Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.
• Depositing Lab: James Naismith
• Publication: Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811. doi: 10.1021/acschembio.8b00050. Epub 2018 Feb 12.

Backbone

• Vector backbone: pJ414
• Backbone manufacturer: DNA2.0
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 6000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: 200 rpm
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Peptide cyclase 1
• Alt name: PCY1
• Species: Saponaria vaccaria
• Insert Size (bp): 2175
• Mutation: WT
• Promoter: T7
• Tag / Fusion Protein:
  • His6-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TAATACGACTCACTATAGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ordered from DNA2.0

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry