pNOC-NLUC
(Plasmid
#98141)
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Purpose(Empty Backbone) Gateway compatible construct for N' terminal fusion or promoter driven NanoLuciferase reporter, hygromycin resistance cassette
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNOC-Flux
- Backbone size (bp) 8793
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Vector typeAlgae, Nannochloropsis
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNOC-NLUC was a gift from Eva Farre (Addgene plasmid # 98141 ; http://n2t.net/addgene:98141 ; RRID:Addgene_98141) -
For your References section:
Identification of circadian rhythms in Nannochloropsis species using bioluminescence reporter lines. Poliner E, Cummings C, Newton L, Farre EM. Plant J. 2019 Jul;99(1):112-127. doi: 10.1111/tpj.14314. Epub 2019 Apr 19. 10.1111/tpj.14314 PubMed 30883973