CMYA5tnC/pDEST26-N-FLAG
(Plasmid
#98130)
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PurposeExpress CMYA5 (aa 3859 – 4069) with a N-terminal FLAG tag in pDEST26 mammalian expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98130 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepDEST26-N-FLAG
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7487
- Total vector size (bp) 8484
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCMYA5
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SpeciesH. sapiens (human)
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Insert Size (bp)642
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GenBank IDNM_153610
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Entrez GeneCMYA5 (a.k.a. C5orf10, SPRYD2, TRIM76)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMYA5tnC/pDEST26-N-FLAG was a gift from Rita Shiang (Addgene plasmid # 98130 ; http://n2t.net/addgene:98130 ; RRID:Addgene_98130)