CMYA5T2/pDEST15
(Plasmid
#98109)
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PurposeExpress CMYA5 (aa 4039 – 4069) Leu allele (4063) with a N-terminal GST tag in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepDEST15
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7013
- Total vector size (bp) 5561
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCMYA5
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Alt nameCMYA5 T allele
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Alt nameCMYA5 Leu allele
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SpeciesH. sapiens (human)
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Mutationrs10043986 (NP_705838.3:p.Pro4063Leu)
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GenBank IDNM_153610
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Entrez GeneCMYA5 (a.k.a. C5orf10, SPRYD2, TRIM76)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer AATGTGCCTGGATGCGTTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMYA5T2/pDEST15 was a gift from Rita Shiang (Addgene plasmid # 98109 ; http://n2t.net/addgene:98109 ; RRID:Addgene_98109) -
For your References section:
A schizophrenia associated CMYA5 allele displays differential binding with desmin. Hsiung A, Naya FJ, Chen X, Shiang R. J Psychiatr Res. 2019 Apr;111:8-15. doi: 10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10. 10.1016/j.jpsychires.2019.01.007 PubMed 30658136