Mouse aB crystallin 250bp promoter/AcGFP
(Plasmid #98102)

• Purpose: Mouse aB crystallin 250bp promoter segment driving GFP expression
• Depositing Lab: Mason Posner
• Publication: Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717/peerj.4093. eCollection 2017.

Backbone

• Vector backbone: pAcGFP1-1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 4374
• Modifications to backbone: None
• Vector type: GFP expressing

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Alpha B-crystallin promoter 250 bp fragment, Mus musculus
• Alt name: Mus Cryab
• Alt name: Cryab
• Species: M. musculus (mouse)
• Insert Size (bp): 259
• GenBank ID: CT010341
• Entrez Gene: Cryab (a.k.a. Crya-2, Crya2, HspB5, P23)
• Promoter: Alpha B-crystallin promoter 250 bp fragment (-259/-1)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: TTCTTTCCTGCGTTATCC
• 3′ sequencing primer: CCATTCACATCGCCATTCA

Resource Information

• Supplemental Documents:
  • Mus 250 bp aB_GFP plasmid map.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry