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Zebrafish aBb crystallin 5kb promoter/AcGFP
(Plasmid #98101)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGFP1-1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4100
  • Total vector size (bp) 9099
  • Modifications to backbone
    None
  • Vector type
    GFP expressing

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Alpha Bb-crystallin promoter 5 kb fragment, Danio rerio
  • Alt name
    Danio Cryabb
  • Alt name
    Cryabb
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    5000
  • GenBank ID
    NM_001002670.2
  • Entrez Gene
    cryabb (a.k.a. cryab2, wu:fe37f08, zgc:91937)
  • Promoter Danio alpha Bb-crystallin 5 kb promoter fragment (-4999/-1)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTCTTTCCTGCGTTATCC
  • 3′ sequencing primer CCATTCACATCGCCATTCA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Zebrafish aBb crystallin 5kb promoter/AcGFP was a gift from Mason Posner (Addgene plasmid # 98101 ; http://n2t.net/addgene:98101 ; RRID:Addgene_98101)

  • For your References section:

    The zebrafish as a model system for analyzing mammalian and native alpha-crystallin promoter function. Posner M, Murray KL, McDonald MS, Eighinger H, Andrew B, Drossman A, Haley Z, Nussbaum J, David LL, Lampi KJ. PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717/peerj.4093. eCollection 2017. 10.7717/peerj.4093 PubMed 29201567
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