Danio alpha Bb-crystallin 1kb promoter/AcGFP
(Plasmid #98098)

• Purpose: aBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos
• Depositing Lab: Mason Posner
• Publication: Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717/peerj.4093. eCollection 2017.

Backbone

• Vector backbone: pAcGFP1-1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 5183
• Modifications to backbone: None
• Vector type: GFP expressing

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Alpha Bb-crystallin promoter 1 kb fragment, Danio rerio
• Alt name: Danio Cryabb
• Alt name: Cryabb
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1074
• GenBank ID: NM_001002670.2
• Entrez Gene: cryabb (a.k.a. cryab2, wu:fe37f08, zgc:91937)
• Promoter: Danio aBb-crystallin 1 kb fragment (-1074/-1)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: TTCTTTCCTGCGTTATCC
• 3′ sequencing primer: CCATTCACATCGCCATTCA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry