Danio alpha Ba-crystallin 3kb promoter/AcGFP
(Plasmid #98097)

• Purpose: aBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos
• Depositing Lab: Mason Posner
• Publication: Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717/peerj.4093. eCollection 2017.

Backbone

• Vector backbone: pAcGFP1-1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 7099
• Modifications to backbone: None
• Vector type: GFP expressing

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Alpha Ba-crystallin promoter 3 kb fragment, Danio rerio
• Alt name: Danio Cryaba
• Alt name: Cryaba
• Species: D. rerio (zebrafish)
• Insert Size (bp): 3000
• GenBank ID: NM_131157.1
• Entrez Gene: cryaba (a.k.a. cryab)
• Promoter: Danio alpha Ba-crystallin 3 kb fragment (-3000/-1)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TTCTTTCCTGCGTTATCC
• 3′ sequencing primer: CCATTCACATCGCCATTCA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry