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PurposeSecond-generation rabies viral vector genome
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98039 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonecSPBN (pSAD)
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Backbone manufacturerKarl-Klaus Conzelmann & Matthias Schnell
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCre
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SpeciesSynthetic
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Insert Size (bp)1056
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRVdGL-4Cre was a gift from Ian Wickersham (Addgene plasmid # 98039 ; http://n2t.net/addgene:98039 ; RRID:Addgene_98039) -
For your References section:
Nontoxic, double-deletion-mutant rabies viral vectors for retrograde targeting of projection neurons. Chatterjee S, Sullivan HA, MacLennan BJ, Xu R, Hou Y, Lavin TK, Lea NE, Michalski JE, Babcock KR, Dietrich S, Matthews GA, Beyeler A, Calhoon GG, Glober G, Whitesell JD, Yao S, Cetin A, Harris JA, Zeng H, Tye KM, Reid RC, Wickersham IR. Nat Neurosci. 2018 Apr;21(4):638-646. doi: 10.1038/s41593-018-0091-7. Epub 2018 Mar 5. 10.1038/s41593-018-0091-7 PubMed 29507411