pGEX-SF3B1-NTerm
(Plasmid #97425)

• Purpose: Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli
• Depositing Lab: Alex Minella
• Publication: Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001654. doi: 10.1074/jbc.RA118.001654.

Backbone

• Vector backbone: pGEX 6P2
• Backbone manufacturer: GE Healthcare Life Sciences
• Backbone size w/o insert (bp): 4985
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Follow protocol provided in GE Healthcare pGEX-6P-2 GST Expression Vector Product Specification Sheet for inducible expression of GST-N-term-SF3B1 and refer to associated handbook on GST Gene fusion system Use a strain such as BL21 for protein expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SF3B1-N-term (AA1-500)
• Species: Synthetic
• Insert Size (bp): 1500
• Mutation: Silent mutation at nt876, aa289 (AGG to AGA)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: CGCGGATCCATGGCTAAGATCGCTAAAACCCACGAGGAC
• 3′ sequencing primer: CCGGAATTCTCACAGCTTCATGATTTTGCGCTCCTTCTGTTCCTCTGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry