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Purpose(Empty Backbone) Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 97423 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEAQselectK
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mismatches in GFP are not a concern for plasmid function; intron in GFP prevents recognition by analytical software.
Addgene Note: Large plasmid may be unstable in DH5alpha. We suggest screening colonies before use.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEff was a gift from Nikolai Ravin (Addgene plasmid # 97423 ; http://n2t.net/addgene:97423 ; RRID:Addgene_97423) -
For your References section:
Efficient Transient Expression of Recombinant Proteins in Plants by the Novel pEff Vector Based on the Genome of Potato Virus X. Mardanova ES, Blokhina EA, Tsybalova LM, Peyret H, Lomonossoff GP, Ravin NV. Front Plant Sci. 2017 Feb 28;8:247. doi: 10.3389/fpls.2017.00247. eCollection 2017. 10.3389/fpls.2017.00247 PubMed 28293244
