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Addgene

Desmin/pAS2-1
(Plasmid #97407)

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Item Catalog # Description Quantity Price (USD)
Plasmid 97407 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pAS2-1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8393
  • Total vector size (bp) 8604
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Desmin
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001927
  • Entrez Gene
    DES (a.k.a. CDCD3, CSM1, CSM2, LGMD1D, LGMD1E, LGMD2R)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer Gal4 N-term
  • 3′ sequencing primer GAL4 3'-BD: 5'-CATAAGAAATTCGCCCGG-3'
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Desmin/pAS2-1 was a gift from Rita Shiang (Addgene plasmid # 97407 ; http://n2t.net/addgene:97407 ; RRID:Addgene_97407)

  • For your References section:

    A schizophrenia associated CMYA5 allele displays differential binding with desmin. Hsiung A, Naya FJ, Chen X, Shiang R. J Psychiatr Res. 2019 Apr;111:8-15. doi: 10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10. 10.1016/j.jpsychires.2019.01.007 PubMed 30658136
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