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Addgene

PEP108E
(Plasmid #97394)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97394 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAMBIA1380
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP1-10OPT
  • Species
    Synthetic
  • Tag / Fusion Protein
    • Plasma membrane target

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene’s sequencing results found an insertion of IS4 transposase in this vector not annotated in the original sequence. The depositor has verified that this vector is functional for target gene expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PEP108E was a gift from Savithramma Dinesh-Kumar (Addgene plasmid # 97394 ; http://n2t.net/addgene:97394 ; RRID:Addgene_97394)

  • For your References section:

    Spatiotemporal Monitoring of Pseudomonas syringae Effectors via Type III Secretion Using Split Fluorescent Protein Fragments. Park E, Lee HY, Woo J, Choi D, Dinesh-Kumar SP. Plant Cell. 2017 Jul;29(7):1571-1584. doi: 10.1105/tpc.17.00047. Epub 2017 Jun 14. 10.1105/tpc.17.00047 PubMed 28619883
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