PEP101E
(Plasmid
#97387)
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Purposebinary construct of cytosolic sfGFP1-10 for plant
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 97387 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAMBIA1380
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP1-10OPT
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene’s sequencing results found an insertion of IS4 transposase in this vector not annotated in the original sequence. The depositor has verified that this vector is functional for target gene expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PEP101E was a gift from Savithramma Dinesh-Kumar (Addgene plasmid # 97387 ; http://n2t.net/addgene:97387 ; RRID:Addgene_97387) -
For your References section:
Spatiotemporal Monitoring of Pseudomonas syringae Effectors via Type III Secretion Using Split Fluorescent Protein Fragments. Park E, Lee HY, Woo J, Choi D, Dinesh-Kumar SP. Plant Cell. 2017 Jul;29(7):1571-1584. doi: 10.1105/tpc.17.00047. Epub 2017 Jun 14. 10.1105/tpc.17.00047 PubMed 28619883