UKNP1.2.1
(Plasmid
#97356)
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PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 97356 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1_V5-His_Topo
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHCV E1E2
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Alt nameUKNP1.2.1
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SpeciesHepatitis C virus
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Insert Size (bp)1734
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GenBank IDKU285152.1
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer bGHR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UKNP1.2.1 was a gift from Jonathan Ball (Addgene plasmid # 97356 ; http://n2t.net/addgene:97356 ; RRID:Addgene_97356) -
For your References section:
A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in Vaccine Research Reveals Extremes of Monoclonal Antibody Neutralization Resistance. Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey T, Irving WL, Ball JK, Tarr AW. J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15. 10.1128/JVI.02700-15 PubMed 26699643
Map uploaded by the depositor.
