pBS3ndd
(Plasmid #97354)

• Purpose: Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant
• Depositing Lab: Jamorn Somana
• Publication: Jamorn Somana Lab Plasmids (unpublished)

Backbone

• Vector backbone: pBluescriptII SK+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2990
• Total vector size (bp): 3446
• Modifications to backbone: Changed ampicillin resistant gene to kanamycin resistant gene
• Vector type: Bacterial Expression ; cloning

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Growth medium may contain glucose but not beta-D-galactopyranoside such as IPTG or lactose. Kanamycin resistant phenotype will be lost if culture at 42 C or above.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nucleoid Disruptive Domain
• Alt name: ndd
• Species: bacteriophage T4
• Insert Size (bp): 456
• Mutation: changed methionine 1 to isoleucine
• GenBank ID: HM137666
• Promoter: E. coli lacZ promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: GCTCTAGAACTAGTGGATC
• 3′ sequencing primer: CGAGGTCGACGGTATC

Resource Information

• Supplemental Documents:
  • pBS3ndd.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry